Bukti serologik infeksi APMV-1 pada babi di Bali (penulis I)

ABSTRACT Avian Paramyxovirus type 1 (APMV-1) is a virus infecting birds with mortality level varies according to its pathotype. Although the virus is normally originated from birds, the recent report showed that it can be isolated from pig. In order to find out APMV-1 infections on pig in Bali, a serologic study was conducted to detect the antibody against APMV-1 among pigs in Bali. Indirect enzyme-linked immunosorbent assay (ELISA) was used to detect the antibody against APMV-1 in pig sera. The serum samples with optical density value over the cut off value (COV) was considered as those containing Ab against APMV- 1. Of 215 samples collected from traditional farms of eight regency and city in Bali namely Negara, Tabanan, Badung, Denpasar City, Gianyar, Bangli, Karangasem and Buleleng Regency, 174 samples (80,93%) were positive antibody against APMV-1. On the other hand, there were 125 (98,42%) positive sample among 127 sample collected from Pesanggaran abattoir. Furthermore, the 8-9 month age pigs showed that their antibody against APMV-1 were higher (p<0.01) than those of 3-6 months age. The ELISA positive samples were further confirmed using Western immunoblotting assay. The anti-APMV-1 antibody positive sera reacted with the virus specific protein band of 258 kDa and the negative sera did not react with the viral protein. As pig has never been vaccinated with APMV-1 vaccine, the presence of Ab against APMV-1 showed that the pigs were naturally infected with APMV-1.