Induction of Apoptosis and Antiangiogenesis Effects of Pinostrobin from Kaempferia pandurata Roxb against Induction of Fibrosarcoma Mice Results Benzopiren

Induction of apoptosis and antiangiogenesis effects of Pinostrobin from Kaempferia pandurata Roxb against Fibrosarkoma mice results benzopiren induction has been done. Examination or surgery begins taking tissue fibrosarcoma in mice infected and weigh fibrosarcoma obtained. Fibrosarcoma tissues were then stored in containers that have contained 10 % formalin. Weighing results showed that the concentration of pinostrobin oral 80 mg / kg can inhibit the growth of fibrosarcoma with a gram weight of 68.62 % and a cancer drug ( control + ) there is resistance 95.95 % compared to the negative control which is only given orally CMC - Na, this means pinostrobin potentially be developed as a cancer chemotherapy. Furthermore done shooting patohistologi tissue fibrosarcoma with HE staining with a light microscope with 400x magnification. The results obtained showed many chromatin (polikromatin) which prove the damage caused by having fibrosarcoma cells. Immunohistochemical assay showed oral pinostrobin concentration 80 mg / kg body weight can increase the expression of p53 to apoptosis induction could take place and the decreased expression of VEGF angiogenesis which proves the existence of barriers. Keywords: Pinostrobin, fibrosarcoma, apoptosis, antiangiogenesis, p53 dan VEGF