Effect Of Euchresta Horsfieldii Lesch Benn Leaf Extract On Increases Enzyme Activity Of Superoxide Dismutase And Glutathione Peroxidase In Rats with Maximum Physical Activity

Maximum physical activity can lead to increased metabolism and oxygen consumption so as to accelerate the formation of ROS. This study aims to determine the effect of the ethyl acetate extract of Euchresta horsfieldii lesch benn leaf on increasing the activity of SOD and GPx in blood plasmawistar rats with maximum physical activity. Of antioxidant capacity in vitro with1,1-diphenyl-2-pycrylhidrazyl where as the in vivo measurement of the enzyme activity of SOD and GPx in Wistar rats. The results showed that the ethyl acetateextract has antioxidant capacity with IC50 value of 393.95 mg/mL and total flavonoid levels of 6619.72 mg QE/100g or 6.62% QE. The extract ethyl acetate 20 mg/200g bw/day provides the best results that can increase the activity of superoxide dismutase as much as 34.93 times (171.44%) and the ethyl acetate extract of 30 mg/200g bw/day can increase levels of glutathione peroxidase were 9,76 times (41.80%). Statistical test results one way ANOVA on the mean activity of SOD and GPx after the administration of ethyl acetate extract showed the p value of 0.001 (p<0.05), so that the value indicates that the five treatment given to Wistar rats provide a significantly different effect.