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Journal article
Molecular Characterization of the Relationshipsamong Amylomyces rouxii, Rhizopus oryzae, andRhizopus delemar
Hideki KITO Ayumi ABE I NENGAH SUJAYA Yuji OD , Kozo ASANO TeruoSONE
Volume : 73 Nomor : 4 Published : 2009, April
Bioscience Biotechnology and Biochemistry
Abstrak
Twenty-one strains ofAmylomyces rouxiiisolatedfrom starters of Asian fermented foods were dividedinto two groups, lactic acid (LA) and fumaric and malicacid (FMA) producers, by organic acid productivity inliquid culture. Phylogenetic analysis based on theldhBgene, ribosomal RNA encoding DNA (rDNA) internaltranscribed spacer (ITS) sequence, and genome-wideamplified fragment length polymorphism (AFLP) re-vealed thatA. rouxiiwas grouped into two majorclusters as to organic acid accumulation, correspondingtoRhizopus oryzaeandRhizopus delemar. These obser-vations suggest that the speciesA. rouxiiis composed oftwo distinct types, derived fromR. oryzaeorR. delemarviadomestication in the starters