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Journal article
Adhesion properties ofLactobacillus rhamnosusmucus-binding factor to mucin and extracellular matrix proteins
Keita Nishiyama Koichi Nakamata Shintaro Ueno Akari Terao Ni Putu Desy Aryantini I NENGAH SUJAYA Kenji Fukuda Tadasu Urashima Yuji Yamamoto Takao Mukai
Volume : 79 Nomor : 2 Published : 2015, February
Bioscience Biotechnology and Biochemistry
Abstrak
We previously described potential probioticLactobacillus rhamnosusstrains, isolated from fermented mare milk produced in Sumbawa Island, Indonesia, which showed high adhesion to porcine colonic mucin (PCM) and extracellular matrix (ECM) proteins. Recently, mucus-binding factor (MBF) was found in the GG strain of L. rhamnosusas a mucin-binding protein. In this study, we assessed the ability of recombinant MBF protein from the FSMM22 strain, one of the isolates ofL. rhamnosusfrom fermented Sumbawa mare milk, to adhere to PCM and ECM proteins by overlay dot blot and Biacore assays. MBF bound to PCM, laminin, collagen IV, andfibronectin with submicromolar dissociation constants. Adhesion of the FSMM22mbfmutant strain to PCM and ECM proteins was significantly less than that of the wildtype strain. Collectively, these results suggested that MBF contribute toL. rhamnosushost colonization via mucin and ECM protein binding.