EN 
IN
Journal article
Protein Profile Analysis of Escherichia coli O157 H7 from Human and Animals Origin
I Wayan Suardana KOMANG JANUARTHA PUTRA PINATIH dr. Ni Luh Ketut Ayu Ratnawati drh. Dyah Ayu Widiasih, Ph.D
Volume : 2 Nomor : 6 Published : 2013, June
International Journal of Current Microbiology and Applied Sciences
Abstrak
Healthy animals especially of the type of ruminants (cattle, sheep, and goats) and pigs are commonly carrying a large number of STEC and Escherichia coli O157:H7 in their feces, so they were considered as a main reservoir of the agent. The transmission of this agent to human commonly by consumption of less cooking meat, unpasteurized dairy product, water contaminated by feces, as well as person to person transmission has also been reported. Based on the importance of this agent to human healthy especially to the epidemiological aspect in order to know the pathogenesis of the agent, the genetic variation study of isolates originated from animals and human needs to be done. The study began by the cultivation of 20 E. coli O157:H7 isolates originated from animals and human, followed by analysis of the chemical characterization using SDS-PAGE (Sodium Dodecyl Sulphonate Polyacrylamide Gel Electrophoresis) method. The data from SDSPAGE were analyzed using simple matching coefficient (Ssm) and algorithm unweighted pair group method using arithmetic averages (UPGMA). The results show that the protein profile of most E. coli O157:H7 local isolates are known to have similarity value more than 70%. These results prove that the isolates of animal origin can be found in humans or nature zoonotic