Application of DNA fingerprinting technology in the conservation of unique species and ecosystem restoration in the Batukahu Nature Reserve, Bali.

The Batukahu Nature Reserve has several unique floras including cemara pandak (Podocarpus imbricatus Blume) and palem tabah (Pinanga arinasae J.R. Witono). Forest fires that occurred in 1994 in Bukit Pohen, which is one of the Batukahu Nature Reserve areas, has changed forest vegetation. Conservation activities of these two species and ecosystem restoration are needed to keep the forest functioning properly. Conservation strategies require the study of population structure and genetic diversity both intra-and inter-population. This information is needed so that the conserved population has a high diversity to avoid inbreeding and decreased genetic integrity. This research generally aims to conserve pandak and palm palms as well as ecosystem restoration in Bukit Pohen and Bukit Tapak Batukahu Nature Reserve based on genetic diversity information. In more detail, the aim is to evaluate the population structure and genetic diversity of Pandak and Palm trees using DNA fingerprinting techniques, so that populations that have priority to be conserved and sources of material for nursery conservation can be determined. This study was divided into two stages, namely (1) determination of population structure and genetic diversity of species of the Nature Reserve, (2) nurseries of target species.