Evaluation and selection of salinity stress tolerance in local Balinese rice cultivars

Local rice varieties are important germplasm in rice breeding. There are still many traditional Indonesian rice germplasm that have not been characterized and underutilized as is Bali's local rice. One obstacle in rice production is environmental factors that are not appropriate. Salinity affects every stage of plant development, but the response of plants differs with each growth. Therefore in the first year the salinity stress level will be observed at the germination level and the seedling phase. Determination of the percentage of germination is carried out on petridish. In the seedling phase hydroponic method will be used with Yoshida nutrient solution with stress levels of NaCl 4dS m-1, 6dS m-1, 106dS m-1 and 12 dSm-1. Observations were made on the character of growth and oxidative stress levels on four Balinese rice cultivars and IR64 as a comparison. Chlorophyll and MDA content were also determined. The expression of antioxidant genes (SOD and Cat) was analyzed by semi-qRT-PCR.