Genetic Characteristics of Four Microsatellite Markers Associated with Birth Weight in Bali Cattle

The aim of the present study was to verify associations between four microsatellite markers with birth weight in Bali cattle. A sample of 42 (including 21 dam and 21 progeny) was characterized for BMS1248, ILSTS006, TEXAN 15 and IGF-1. Associations between the genetic markers and birth weight were analyzed. The genotypes were classified according to allele size. Fragments of different sizes was different allels which named A-E and so on, respectively according to the order size of allel sequence. Four microsatellite markers were selected to analyze the genomic DNA of 42 Bali cattle The results showed that a total of 162 different alleles were found and the number of alleles in each locus was 2 to 6. The DNA fragment length was 100 bp to 444 bp and the number of mean valid alleles was 1.3069 to 4.2288. The value of heterozygosity was 0.2361 to 0.7677 and the mean polymorphism information content (PIC) was 0.5368. A GLM procedure was used to analyze the effects of these four microsatellites on birth weight. The BMS1248 had a significant impact on birth weight.These results suggest that microsatellite is a potential DNA marker for birth weight trait in bali cattle.