Correlation Analysis of Microsatellite DNA Markers with Body Size, Lenght and Height of Bali Cattle

Abstract: Forty one Bali cattle and 3 microsatellite DNA markers(BMS1248, ILSTS066 and IGF-1) were used to study the population genetic variation Bali cattle and the relationships between the gene loci and their body size, lenght and height traits.A GLM procedure was used to analyze the effects of these 3 microsatellites on body weight, length and height. The results showed that a total of 10 different alleles were found and the number of alleles in each locus was 2 to 6. The DNA fragment length for BMS1248 loci, ILSTS066 and IGF-1 were 120-180bp, 120-170 bp and 210-230 bp respectively.Mean values for observed heterozygosity for ILSTS066, BMS1248 and IGF-1 were 0.927, 0,561 and 1 respectively. The PIC value at the BMS1248 loci demonstratedhigh polymorphism in this population with PICvalues 0.6090. The microsatellite loci BMS1248 had a positive correlation with body weight and body lenght. While for ILSTS066 and IGF-1 loci had a negative correlation with body weight, length and height. Key words: Microsatellite Body size Body height Body lenght Bali cattle