isolation, Characterization and Antibacterial Activity of Triterpenoid Compounds Fraction Chloroform Bark Phyllanthus Niruri Linn

ABSTRACT The use of traditional medicine as Phyllanthus niruri compounds have been reported to contain triterpenoids, alkaloids and flavonoids are efficacious as a cure dysentery, rheumatic, anti-inflammatory and intestinal worms in children. This study aims to isolate, characterization and determine the antibacterial activity of the triterpenoids compounds of chloroform fraction bark of Phyllanthus niruri Linn. A total of 700 g of dry powder of bark Phyllanthus niruri Linn macerated with n-hexane for 24 hours. Subsequently extract thick n-hexane in the partition with chloroform produce a yield of 12.446 (1.778%). The results showed that isolates compounds Fi chloroform fraction bark contains compounds Phyllanthus positive niruri triterpenoids with Rf 0.411, and is able to inhibit the growth of bacteria Staphyloccocus aureus at a concentration of 1000 ppm with inhibition of 12 mm. Identification using a spectrophotometer mass and HNMR showed that the compound isolates F1 possibilities include compound group triterpene steroids with the characteristics of the molecular ion m/z 424 [M +], m/z 381 [M+-C3H7], m/z 341 [M+- C3H4], m/z 313 [M+- C2H4], m/z 287 [M+-C2H2], 229 [M+- C3H6O], m/z 151 [M+-C6H6], m/z 109 [M+ –C3H6], m/z 95 [M+- CH2], m/z 81 [M+- CH2], m/z 69 [M+-C], m/z 55 [M+- CH2], m/z 43 [M+-C], and a singlet peak ? 7.337 [aromatic], multiplet ? 0.808 to 1.682 [CH2 and CH3], doublet 5.8 ? [C = CH], doublet 4.6 ? [OH]. Key words: Isolation, Characterization, Antibacterial, Triterpenoids, Phyllanthus niruri Linn