Detection of Pathogen Potential Bacterial in Porcine Respiratory Disease Complex

INTRODUCTION

 Pig livestock on the island of Bali is the most desirable livestock by people who are Hindu and are one of the leading in the Faculty of Veterinary Medicine, Udayana University. A common obstacle is the presence of a complex respiratory tract disease known as porcine respiratory desease complex (PRDC). The disease is caused by bacteria and viruses, so it is known as multimicrobial desease. Potentially pathogenic bacteria are Mycoplasma hyopneumoniae, Actinobacillus pleuropneumoniae, Actinobacillus suis, Bordetella bronchiseptica, Haemophilus parasuis, Streptococcus suis and Salmonella cholerasui, Pasteurella sp, Streptococcus sp, Staphylococcus sp, Klebsiella pneumonia and Escherichia coli (Bochev, 2007; Lecturer et al., 2007 ; Loera-Muro et al., 2015). Loera-Muro et al. (2014) PRDC causes disruption to growth, economic loss and death. The first new disease incidence in Indonesia was reported by Priadi, et al. (2004) caused by Haemophilus parasuis isolated from the lungs of infected pigs. Reports of the incidence of diseases in pigs in Bali still exist but the results of the study have not been reported

RESEARCH METHODS

The study sample consisted of clinical nasal and subclinical piglets of PRDC. Isolation and bacterial identification are carried out by conventional methods (Cowan, 1974; Carter, 1984). The bacterial isolates were then tested for their sensitivity to antibiotics by disc diffusion method according to Kirby-Bauer (Koneman et al, 1984).

Table. Distribution of Research Sample Amount

 RESULTS AND DISCUSSION

The bacterial isolates isolated from pigs with clinical PRDC include: Staphylococcus sp beta hemolytic non-coagulase, Streptococcus sp alfa hemolytic non-coagulase . Distribution of bacterial isolates was obtained from clinical pigs at the time of weaning and after weaning. Bacterial isolates isolated from subclinical pigs were Staphylococcus sp, Streptococcus sp and Escherichia coli. In this study, isolates of Staphylococcus sp beta hemolytic non-coagulase and alpha hemolytic Streptococcus sp non-coagulase were suspected to be the causative agents of PRDC. According to Cowan (1974) and Carter (1984) hemolysin toxin is a bacterial virulence factor. Sensitivity test results showed that Staphylococcus sp non kougulase and E. coli were sensitive to Streptomycin, Kanamycin, Clindamycin, Bacitracin and Doxycycline. Similar results were also shown by Streptococcus sp alpha hemolytic non-coagulase, except resistant to doxycycline.

CONCLUSION

The results of this study concluded that bacterial isolates isolated and suspected as agents causing PRDC were beta hemolytic Staphylococcus sp non-coagulase, alpha hemolytic Streptococcus sp and E. coli. These bacterial isolates are sensitive to Streptomycin, Kanamycin, Clindamycin, Bacitracin and Doxycycline, except Streptococcus sp. alpha hemolytic noncoagulase resistant to doxycycline

BIBLIOGRAPHY

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