Study on Genetic Markers and Cloning of Shiga Like Toxin-2 Escherichia coli O157: H7

The results show  that the prevalence of  E. coli O157:H7 infections in all samples is 6.4%. Genomic analysis of all E. coli O157:H7 isolates by            PCR-RAPD method shows  that  the isolates are known to  have a similar value ranging from 75.1 to  96.6% and  it also  shows  two  specific bands, 1721 and 700 bp, known  as a marker to identify  the E. coli O157:H7 isolates originated from healthy and unhealthy cases. The analysis of virulence marker shows that KL48(2) isolate originated from human and SM25(1) isolate  originated from cattle are known to bear eae, stx1 and stx2 genes as equal as E. coli ATCC 43894 control.  Although the study of the variation of q gene sequences,  promoter sequences, and Ribosome Binding Site (RBS) of KL-48(2) and SM25(1) indicates similar sequences, they have a different  level expression of Stx2.  In addition, through genetic engineering, the stx2 gene can be cloned  in cloning vector and  expression  vector, and  recombinant protein as a result of genetic engineering  is known to have  a good potency to use  as an immunodiagnostic  agent and vaccine candidate