Intake of Flavonoid Glycosides of the Grapefruit Extract (Henslowia Frutescens Champ) Inhibits the Expression of Breast Cancer Cells in Benzo-alpha-induced Wistar

               Breast cancer is a malignant tumor that forms in breast tissue. Malignant tumors are a collection of cancer cells that develop rapidly into the surrounding tissue or spread to goto body even further. WHO data show that 1.2 million people have been diagnosed with breast cancer in women. This disease is greatly feared by the public and is ranked second cause of death after cervical cancer. The aim of this study is to isolate and prove the intake of flavonoid glycosides extract of orange parasite leaves (Henslowia Frutescens Champ) has the potential to inhibit the formation of breast cancer cells in wistar rats after being induced benzo-α-pyrene.

               This research is purely experimental with post test only control group design. A total of 50 wistar rats used in this study were divided into 5 (five) groups while the benzo-α-pirene compounds were used to induce breast cancer in mice. Citrus parasite extract is given when inoculating doses of 100 mg / kg, 200 mg / kg, and 300 mg / kg for 3 weeks and after the onset of tumors with 2 multilevel doses. The emergence of tumors was observed by palpation and observed under a microscope, and stained with HE staining. Data obtained from all groups were processed with the SPSS 19.0 program performed a non-parametric Kruskal Wallis statistical test and continued with the Mann-Whitney test.

               The results of this study are expected to find bioactive compounds flavonoids glycosides from orange parasite extract (Henslowia Frutescens Champ) as candidates for anticancer breast herbal medicine with an indication of decreasing proliferasisel tumor activity and an improvement in breast cancer cell tissue by immunohistochemistry