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IN
Journal article
Characteristic of Bola DRB3.2 Gene in Taro Cattle
I GEDE SOMA I NYOMAN MANTIK ASTAWA I DEWA MADE SUKRAMA I NENGAH WANDIA
Volume : 18 Nomor : 1 Published : 2017, January
Global Veterinaria
Abstrak
Abstract: BoLa DRB3.2 gene plays an important role in im catlle. BoLA DRB3.2 gene encodes the1unit of MHC mo initiates the immune response. This research aimed to ex Taro cattle. Fifteen blood samples of Taro cattle were taken with 5% EDTA. The DNA was extracted and isolated from DRB3.2gene was amplified using primer F: 5’ATCC TTTAAATTCGCGCTCACCTCGCCGCT-3´. The PCR produ using MEGA 5. The results showed that the BoLA DRB3.2 gen 5’, 267 bp of exon 2 and 18 bp of intron 3’. Nucleotide translat acids. Along the sequences there were 2 sites of single nucl which producing 3 haplotypes of BoLA DRB3.2 gene in Taro is a substitution at amino acid 24R/A/T that make a few cha that the polymorphism of BoLA DRB3.2 gene in Taro cattle i Key words: Taro Cattle Characteristic Bola DRB3.2 Gene