Journal article

CD4 count from cryopreservation of buffy coat and pheripheral blood mononuclear cells

Rasmaya Niruri Inna Narayani Wayan Tunas Artama I NYOMAN MANTIK ASTAWA Ahmad Hamim Sadewa

Volume : 2 Nomor : 2 Published : 2015, April

International Journal of Biological Sciences and Biotechnology

Abstrak

This study aimed to determine CD4 count from cryopreservation of Buffy coat (BC) and Peripheral Blood Mononuclear Cell (PBMC) with and without ficoll. Fifteen EDTA Blood sample (2 ml for each tube) were drawn from one adult healthy subject. The samples were categorized into five group before measuring the CD4 level (which were fresh whole blood [Group(G)-I], BC without ficoll [fresh and frozen ] , and PBMC resulted from BC and ficoll isolation [fresh and frozen ]. Each group was replicated three times. Blood storage before preparation was less than four hours. Two months cryopreservation using liquid nitrogen (in 40% FBS, 10% DMSO, and RPMI) was conducted. The mean value of CD4 count (cell /?l) were 522 (G-I), 1410 (G-II), 906 (G-III), 807 (G-IV), and 733 (G-V). CD4 count, after 2 month preservation in liquid nitrogen, of the BC sample (G -III) was higher (906 cell /?l ) than PBMC (G-IV) sample (733 cell /?l). Keywords: CD4, Buffy coat, PBMC, Cryopreservation

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