PENGARUH KOMBINASI JENIS PELARUT DAN PERBANDINGANNYA TERHADAP KARAKTERISTIK EKSTRAK BUAH PANDAN (Pandanus tectorius)

Pandan plant (Pandanus tectorius) grow well at the coast land of many tropical countries. The ripe fruit of the pandan plant has an oval shape, red color, and contain bioactive compounds, such as carotenoid. Extraction process is required to find the natural extract of the fruit. The aims of this study was to find out the effect of the solvent type combination and their ratio on the characteristics of pandanus fruit extract. This study was experimental study, which was designed by randomized block design. The experiment was conducted as a factorial experiment, which consisted of two factors. The first factor was the solvent type combination (acetone:chloroform and acetone:ethanol) and the second factor was the ratio of solvent (1:1; 1:2; and 1:3). The best solvent combination and its ratio for producing the extract was determined by effectiveness test. The results showed that the solvent type combination affected the yield, the total carotenoids content, the level of brightness (L*), and the level of redness (a*) but did not affect the level of yellowness (b*). The ratio of solvent combination affected the yield but did not affect the total carotenoid content, the level of brightness (L*), the level of redness (a*), and the level of yellowness (b*). The combination of acetone and ethanol with ratio of 1:3 was the best treatment to produce pandanus fruit extract with the yield of 3.13%; total carotenoid content of 12.72%; the level of brightness (L*) 11.11; the level of redness (a*) 3.81; and the level of yellowness (b*) 31.48.