Induksi Organogenesis pada Mikropropagasi Anggrek Vanda tricolor Lindl. serta Deteksi Variasi Genetik Hasil Perbanyakan dengan RAPD

ABSTRACT Induction of Organogenesis on Micropropagation of Vanda tricolor Orchid and Detection of Genetic Variation of the Progenies Using RAPD Marker. Research concerning on micropropagation of Vanda tricolor orchid and detection of genetic variation among the progenies has been conducted in the year of 2015. The objective of the research was to obtain the most appropriate explant type for inducing organogenesis and to observe genetic variation of the progenies using Random Amplification of Polymorphic DNA (RAPD). Various pieces of organ from 18 month old-seedlings were used as material of micropropagation, i.e. shoot tip, shoot base, leaf tip and leaf base. Explants were planted on MS medium without plant growth regulator. The percentage of explant producing shoots and the average number of shoots per-explant were observed at 12 weeks after planting. Progenies resulted from the micropropagation were then analysed using RAPD with 5 primers, i.e. OPA8 (5’ GTGACGTAGG 3’), OPA9 (5’ GGGTAACGCC 3’), OPA10 (5’ GTGATCGCAG 3’), OPA11 (5’ CAATCGCCGT 3’), OPA13 (5’ CAGCACCCAC 3’). The results showed that the most appropriate explant for inducing organogenesis was shoot base. Analysis of RAPD showed that the slight variation was detected between mother plant and the progenies and also among the progenies. The results obtained from the current research are promising and suggests that RAPD markers can be utilized as a simple molecular tool to assess the genetic variation of plants derived in-vitro. Key words : Organogenesis, Vanda tricolor orchid, genetic variation, RAPD