Simultaneous detection of ompW and ctxA gene of Vibrio cholera isolated from ice used for marine product preservative by Duplex Polymerase Chain Reaction assay (dPCR)

Background: Cholera is an infectious disease with symptom rice water stool diarrhea caused by Vibrio cholerae. Cholera transmitted by water or contaminated seafood. Ice usually used as a preservative marine products which vulnerable to contamination. Objective: This study aimed to determine the bacterial contamination of V. cholerae carrying the ompW and ctxA genes on ice preservatives marine product in Kedonganan Fish Market, Badung-Bali. Method: This study used 3 samples of ice that has not been used to preserve marine products and 20 samples of ice that has been used to preserve marine products. Samples were examined using bacterial culture methods and followed by dPCR assay to detect ompW and ctxA genes. Result: The results showed all samples of ice that has not been used preservative marine products are not contaminated by V. cholerae, while 16 of 20 (80%) samples of ice that had used contaminated by V. cholerae based on ompW gene and found no samples that carry ctxA gene. Conclusion: Ice agent (EA) was not contaminated by V. cholerae, while use ice sample marine product preservative contaminated with V. cholerae with 80% positive and none of isolates carry the gene ctxA. Keywords: Vibrio cholerae, ompW, ctxA, duplex polymerase chain reaction