Selection of Saccharomyces Spp Isolates (Isolation from Colon Beef of Bali Cattle) as Probiotics Agentand Colon Cancer Preventionandits Effect on Pollard Quality as Feed

This experiment was carried out to study probiotics properties and colon cancer prevention of Saccharomyces spp isolation from colon beef of Bali cattle samples can be used to alleviate the negative effect of pollard as feed. Three isolates of Saccharomyces spp (Sc.SB-3; SB-6; and SB-7) were isolated from colon beef of Bali cattle samples in the first experiment. The whole isolate of Saccharomuces spp showed resistant growth in different temperature (100-450C) andacid conditions (2.5-4.5). However, only two isolates (Sc.SB-6 and SB-7) had potential as probiotics sources (resistant on both acid and bile salt, and couldprevent colon cancer). The study showed that fermentation of pollard using of Saccharomyces spp.SB-6 and SB-7 culture could improve significant differences (P<0.05) on digestibility of its dry matter (DM), organic matter (OM), crude protein (CP), crude fibre (CF), and increased its metabolizable energy of pollard. Fermentation caused increase of crude protein (CP) and crude fibre (CF) of pollard.It was concluded that two isolates (Sc.SB-6 and SB-7) were isolated from colon beef of Bali cattle samples.They were potential as probiotics sources and colon cancer prevention.Their utilization in pollardfermentation could increase nutrient composition and digestibilities. Key words: Saccharomyces spp., Colon Cancer, Probiotics, Digestibility and Pollard.