Effects of Pranajiwa Seed Extracr Repair Damage Pancreatic B Cells throught decreease of Blood Glucose Level, Advanced Glycation end Product and Profile Histopathology in Hyperglycemic Rats

Increased production of free radicals is one factor causing the onset of hyperglycemia. Chronic hyperglycemia increases the formation of reactive oxygen species (ROS), causing oxidative stress. This study aims to determine the effectiveness of seed extract in fixing the rate of ?-cell pancreas damage in hyperglycemia rat wistar induced with alloxan. The study applying a randomized pre-and posttest control group design with dose of pranajiwa seed extract. A total of 40 Wistar rats were used in this study. Rats were divided into four groups: one control group and three treatment groups with different doses of pranajiwa seed extract respectively 0.5 mg/kg bw, 2 mg/kg bw, and 5 mg/kg bw. All control treatment groups have alloxan induced at a dose of 125 mg/kg bw to obtain hyperglycemia. The results showed that, intake of glibenclamide in positive control group blood glucose level to 110,76 mg/dL. For treatment group on the other hand, intake seed extract at P1 reduced blood glucose level to 138.59 mg /dL, at P2 to 128.39 mg/dL and at P3 to 103.69 mg/dL respectively. The mean levels of AGEs in the positive control group (glibenclamide) was 0.049 mol/L, the treatment group was 0.089 mol/L for P1, 0.067 mol/L for P2, and 0.038 mol/L for P3. The mean reduction levels of blood glucose and AGEs, were significantly different (p<0.05). It can be concluded that the administration of pranajiwa seed extract at a dose of 5 mg/kg bw can reduced the rate of destruction of ?-cell damage and structural changes of pancreatic ?-cells tissue histopathology in rats wistar hyperglycemic patients can be improved. Keywords: Pranajiwa, Blood glucose, AGEs, Histopathology and Hyperglycemia