Detection of Streptococcus sp pathogen in pigs

Pigs on the island of Bali become one of the important livestock choices that are cultivated for the purpose of religious and customary activities, sustaining the family's economy and commercial purposes. One obstacle that often arises in pigs is a disease that causes economic loss and death. The most common diseases that occur in pigs are stretococcosis and porcine respiratory desease complex or PRDC. Streptococcal outbreaks in pigs in Bali occurred in 1994 caused by beta hemolytic Streptococcus (Darma, et al., 1994). Next came the PRDC which is also known as multimicrobial disease where Streptococcus sp as one of the causative agents. In general, Streptococcus sp pathogens produce hemolysin toxins that cause blood hemolysis (Carter, 1984). Clinical symptoms of the disease are difficult to distinguish. The typical symptoms are exudate from the nasal cavity, incoordination of motion, tremors in the head area, erythema of the skin and diarrhea. According to Lecturer et al (2007) Streptococcus sp isolated from exudate nasal cavity / trachea, tonsils, feces and organs of patients.

This study aims to detect bacteria by isolating and identifying the haemolytic and biochemical properties of pathogenic Streptococcus sp isolated from infected pigs. The research sample was swab nasal exudate / trachea, feces or organ of the patient taken aseptically. Isolation and identification of bacteria refers to the method of Carter and Wise (2004). Bacterial isolation was carried out by culturing samples on blood agar, then bacterial isolates were identified macroscopically and primary tests included Gram staining, catalase tests and oxidase tests. Furthermore, a physical physicochemical test and biochemical test using API 20 STREP (Biomeriaux) are used to determine the bacterial species. The pathogen isolate of Stretococcus sp was subsequently tested for sensitivity to several antibiotics by the disc diffusion method according to the Kirby-Bauer method (Koneman et al., 1994). The purpose of sensitivity testing is to obtain antibiotics that are effectively used in tackling the disease.

Keywords: pigs, streptococcosis, PRDC, Stretococcus sp pathogen, isolation, identification, sensitivity tes