Characterization and Genetic Identification of Potential Yeast Isolate IS 258 as a Starter of Ethanol Fermentation

This research is aimed for the final characterization and genetic identification of potential local yeast IS 258 which was found as a starter of bioethanol fermentation as a renewable alternative fuel. Previous research has found optimal living conditions, growth character, and early identification of superior isolates IS 258. In this study will be continued on survival in various ethanol levels, tested isolate preference towards oxygen, and fermentation in local sap in Bali to see the ability of IS 258 fermentation in the real world, genetic identification will be carried out to finally determine strains of IS 258 yeast isolates. In the future, IS 258 isolates are expected to be energized with cellulolytic bacteria obtained from previous studies to ferment reducing sugars to produce bioethanol at low prices. Based on the fermentation characterization at various temperatures that have been carried out, IS 258 isolates can be developed to be able to improve the efficiency of bioethanol production from low-grade sugars with fermentation conditions in the tropics.

The research method that will be carried out is: IS 258 isolates were grown on thioglycolic media to determine the preference for oxygen, grown on media with various ethanol concentrations to determine resistance to the ethanol produced, and then to be tested in various kinds of roomie to find out the best roomie , then morphology was identified with an electron microscope and identified by biomolecule (genetic sequencing) to determine the species and strains. The specific target to be achieved in this research is to know the characteristics of IS 258 potential isolates in fermentation in the real world, as well as genetic identification in order to apply for patents.