POTENTIAL OF EXOPHYTIC AND ENDOPHYTIC FUNGUS IN CONTROL OF MANGO ROT DISEASE (Mangifera indica L.)

ABSTRACT

Pathogens that are found to cause fruit rot disease in mangoes are Aspergillus niger and Lesiodiplodia theobromae. Exophyte microbes found in healthy mangoes include: Rhizopus sp. the number of colonies was 24 x 10² cfu, while Nucordia sp., A. flavus, and A. niger with colonies of 18 x 10² cfu, and Streptomyces sp. with a colony of 12 x 10² cfu. Endophytic fungi found only Aspergillus sp. with a 15 x 10² cfu colony. The diversity index obtained from exophyte microbes was 2.9993 and the dominance index was 0.78125. While the endophytic microbial diversity index cannot be analyzed because only one is Aspergillus sp. The highest inhibitory microbial inhibition test results for A. niger were obtained from Rhizopus sp. 1 and Rhizopus sp. 4 when 3 days after inoculation and 7 days after inoculation.  Likewise with pathogens (L. theobromae) both by the same microbial exophytes. The results of antagonistic inhibition test on pathogens (L. theobromae) in vivo obtained the highest by the treatment of C (Rhizopus sp. 3) which was very significantly different from K + P (control with pathogens), while the results of inhibition testing in vivo antagonists against pathogens (A. niger) is the highest C treatment (Rhizopus sp. 2) and very significantly different from the K + P treatment (control with pathogens).

Keywords: fruit rot disease, Asergillus niger and Lasiodiplodia theobromae, molecular identification, and in vitro and in vivo tests.