IDENTIFICATION OF MOLECULAR CAUSES OF SUGAR-APPLE FRUIT DISEASE (Annona squamosa L.) AND ENVIRONMENTALLY FRIENDLY CONTROLLES

Fruit rot disease in sugar-apple plants, is the main cause of fruit damage in sugar-apple cultivation in Bali province. Symptoms of illness begin with black marks on the skin of fruit, which gradually spread throughout the fruit. The result of gene amplification of the internal transcribed spacer (ITS) region of ribosomal DNA (rDNA) using 1.2% agarose gel with TAE buffer 1x at 50 volts for 30 minutes. The DNA bands seen above the UV transilluminator as seen on electroferegram, produce a 600 bp DNA fragment. The cause of the illness was identified using DNA sequences and matched with GenBank ie Lasiodiplodia theobromae, with a percentage of 100% resemblance. The phylogenetic tree of one class pathogen with Lasiodiplodia theobromae.