MAKING PLANT SEEDS IN CVPD DISEASE CONTROL IN SUSTAINABLE ORANGE CULTIVATION

Making citrus plant seeds healthy or disease-free CVPD (Citrus Vein Phloem Degeneration) is needed in order to prevent and control disease development in citrus cultivation in a sustainable manner. The spread of disease through infected plant seeds is the biggest contributor in spreading CVPD diseases from one area to another while spreading through vector D. insects. Citri only transfers from one plant to another at one planting location. Besides that, in the presence of citrus seedlings the presence of pathogens that cause disease is very difficult to detect, because the seeds of plants do not show symptoms of disease. CVPD disease symptoms at all age levels show chlorosis symptoms in leaf lamina and leaf bone color remains green, while in plants over two years old leaves become smaller, stiffer, thicker, fruit smaller to the size of a table tennis ball when compared with healthy plant leaves and eventually the plants die. The research will be conducted on citrus plantations in Rendang District and the Laboratory of Agricultural Biotechnology, Faculty of Agriculture, Udayana University, Denpasar. The research aims to make citrus seedlings free of CVPD disease. Total DNA isolation was carried out to confirm the presence of pathogens that cause CVPD disease in citrus plants in parent trees and plant seedlings using the PCR Technique method using a specific premature of 16S rDNA (f: 0I1 and r: 0I2c) and the results of DNA amplification were extracted at 1% gel agarse. The results of this study will produce recommendations for CVPD disease-free citrus seedlings, which are useful for preventing and controlling CVPD disease early in the planting location.