Serum Estrogen Hormone Levels and Uterine Histological Structure Changes in White Rats Given Red Calliandra Leaf Extract

This study used 24 white female Wistar rats aged 54 days divided into four treatment groups, namely one negative control and three doses of C. calothyrsus leaf extract. The parameters observed were blood estrogen levels and the weight and histological structure of the uterus wall (uterine diameter and thickness of the endometrium, myometrium and perimetrium) of female rats. Effective dosage of C. calothyrsus was 70 mg/kg bw leaf extract, which could increase estrogen hormone levels but did not decrease uterine weight, and still maintained the diameter and thickness of the endometrium, myometrium and perimetrium layers in prepubertal female rats (remain normal as the control group).