A study of the Utilization of Calliandra Leaves and Pineapple Peels in the Feed on Biological Responses, Growth and Reproductive Ability during the Growth Phase in vivo

The research is a grant for two year studies (2015-2016). The first year (2015) was carried out on feeding trial on rats, while the second year (2016) was feeding trial on rabbits. Calliandra plants and pineapple peel by product had negative effects that could be detrimental if directly tested on livestock so that they were tested on experimental animals first. The results of the first year studies showed a decrease in glucose levels and an increase in blood SGPT (ALT) levels in the treatment of 25% calliandra in the feed. In male rats, an increase level of pineapple peel in the feed decreased the thickness of the seminiferous tubule (faster spermatozoa maturation) and decreased testosterone levels (still within normal range). In female rats, the highest increase of estrogen obtained from treatment 25% calliandra + 13.05 g/ rat/ day of pineapple peel. Otherwise, the level of progesterone decreased with increasing levels of pineapple peel in the feed. The results of the second year studies showed that 30% of calliandra leaves + 30% of pineapple peels in the feed showed the highest levels of total blood protein, while blood glucose and cholesterol levels remained normal in rabbits. All feed treatments did not affect the male rabbits reproduction both to testicular histology and testosterone levels. While in the females, histology of the ovaries showed the corpus luteum (luteal phase) and a decrease in estrogen levels with increasing levels of calliandra in the feed.