ULTRAVIOLET RADIATION ON Salmonella sp. BACTERIA AND HEMATOLOGICAL EXAMINATION IN DUCK INFECTED WITH SALMONELLOSIS

Funding period : 2018-2018 Deactivate

Abstrak

Ducks are one of the most popular livestock in Indonesian society. Ducks are mostly carried out extensively. This way can cause ducks easily affected by disease and can spread quickly. One of the diseases that mostly attacks ducks is Salmonellosis caused by Salmonella sp. One way to kill Salmonella sp is by using ultraviolet (UV) light. The purpose of this research is to find the right UV irradiation method to weaken the Salmonella typhimurium bacteria which will be used as a material for making Salmonellosis vaccine and see the hematology of infected ducks.

Salmonella sp bacteria were isolated directly from ducks by cloaca swabs, then put into Selenit Broth media and then cultured. The culture was poured on a petri dish, some were as a control and some were treated with UV irradiation with different times of 5 minutes, 10 minutes, 15 minutes, 20 minutes, 25 minutes, 30 minutes and 35 minutes, with the same distance of 10 cm . The growing colonies are then counted. To see the effect of Salmonellosis disease on physiological ducks infected with Salmonella sp bacteria can be seen from the complete hematology of ducks that can indicate the level of infection that occurs.

 The results showed that Salmonella sp was carrier in ducks because ducks did not show symptoms of pain but when examined microbiologically showed positive containing Salmonella sp. The effective UV irradiation time is 25 minutes where it effectively kills Salmonella sp. Balkteri total growth from the first week to the third week showed a decrease in the number of bacteria. Hematologic features of ducks show that white blood cells and lymphocytes increase in number from the second week to the third week after administration of UV-exposed Salmonella bacteria.