Biosynthesis and Characterization of Silver Nanoparticles (AgNPs) Using Sambiloto Plant Extracts (Andrographis Paniculata) And Potential Antibacterial Activity

BIOSYNTHESIS OF SILVER NANOPARTICLES USING SAMBILOTO LEAF EXTRACT: OPTIMIZATION PROCESS AND CHARACTERIZATION. It has been synthesized silver nanoparticles (AgNP) with biological method or biosynthesis, successfully. The synthesis is done using extract of Sambiloto leaf (Andrographis paniculata Ness). The study involved determining the optimum ratio of synthesis e.g. AgNO3 solution:extract solution and characterization of nanoparticles formed. The characterization included UV-Vis spectrophotometer, TEM, XRD and FTIR spectrophotometer. The results obtained were, the optimum ratio of synthesis is 10 µl:10 ml. The characteristics of AgNP formed are the Surface Plasmon Resonance (SPR) absorbance at a wavelength of 423 nm. The diffraction peaks observed at angles 2q of 38.180, 45.810 and 64.870 which corresponded to the hkl (1 1 1), (2 0 0) and (2 2 0). These results showed that the crystal structure of AgNP formed is face center cubic (FCC) with lattice parameter of 4.03 Å. TEM characterization showed that the size of AgNP nanoparticles was 10-30 nm.