CYP2E1 Gene Identification on Acute Lymphoblastic Leukemia Children in Sanglah Hospital

Acute lymphoblastic leukemia (LLA) is a type of leukemia in which lymphoblasts are present in large quantities in the blood and bone marrow. The results of previous studies indicate that LLA is the most hematological case in Bali and some patients have a background of exposure to toxic materials in the environment. Cytochrome P450 variant CYP2E1 is an important member of the CYP superfamily, which is involved in the metabolism and activation of toxic compounds.

The purpose of this study is to identify the CYP2E1 gene variant in pediatric patients with LLA and findout whether the presence of genetic polymorphisms is related to the environmental situation experienced by patients. The samples used were blood and / or bone marrow samples from the LLA and control groups. The LLA group is pediatric patients (0-18 years) with LLA at Sanglah Hospital in Bali. As a control group are children (0-18 years) who do not have cancer. The method used in the first year to identify the CYP2E1 variant was PCR RFLP, electrophoresis, and PCR product sequencing.

The study began by identifying the CYP gene by amplifying the CYP2E1 gene variant in a sample of buffy coat or bone marrow preparations in pediatric patients with acute lymphoblastic leukemia (LLA). The method used is PCR RFLP using a pair of primers: 5 '- CCA GTC GAG TCT ACA TTG TCA - 3' and 5 '- TTC ATT CTG TCT AAC TGG - 3'. The PCR results were cut with the Pst1 enzyme then sequenced and carried out homology tests into a database contained in the NCBI (National Center for Biotechnology Information).

This research is a laboratory exploration conducted at the Integrated Biomedical Laboratory of the Faculty of Medicine, Udayana University.