Anticoagulant activity test of earthworm extracts from organic agricultural land in Bali in vitro and in vivo

    Bleeding and blood clots in the body are regulated by a complex process known as homeostasis. An imbalance in the homeostasis system can cause thrombus (blood clots) to form. Antithrombotic drugs include anticoagulants needed for the treatment of thrombus, but in general these drugs can pose a risk of bleeding, there is a great likelihood of interactions with other drugs, and the risk of allergies. Earthworms (Lumbricus rubellus) is one of the natural ingredients that contains lumbrokinase compounds and antioxidant polyphenols which may have anticoagulant abilities. The purpose of this study was to examine the anticoagulant activity of earthworm extracts (ECT) from organic agricultural land in Bali.

    This research was conducted in Vitro and in Vivo. In vitro tests used healthy human blood samples from 30 respondents who were divided into 5 groups namely Control + (EDTA), control -, ECT 50%, ECT 75%, ECT 100%. The output of the in vitro anticoagulant test is the measurement of aPTT (Activated Partial Thromboplastin Time), PT (Protrombine Time). In vivo test used 30 rat samples divided into 5 groups: control (+), control (-), three earthworm extract treatments each with a dose of 50 mg / kgBW, 100 mg / kgBW, and 200 mg / kgBW given for 28 days. The outputs of in vivo anticoagulant tests are Bleeding Time (BT) and Clothing Time (CT). The results of the study will be analyzed using SPSS. The expected outcome of this research is the publication of articles in the DOAJ national journal.