Effect of Ethanolic Purple Sweet Potato Tuber (IPOMEA BATATA L.) of Ki67 mRNA Expression in Vaginal Tissue of Bilateral Ovariectomized Wistar Rat

Vaginal atrophy is a problem that is often experienced by post-menopausal women. This condition is characterized by thinning of the vaginal epithelium which is lowered by a decrease in estrogen hormone levels. Phytoestrogens are an alternative to estrogen for vaginal atrophy therapy because they show higher effectiveness and do not increase the risk of using cancer. Phytoestrogens given to animal models of menopause as well as post-menopausal women are able to improve the symptoms. Phytoestrogens have a structure similar to estrogen so that they are able to attach to estrogen α and β receptors on vaginal epithelial cells, which are involved in regulating the rate of epithelial cell proliferation. Ki67 is a marker of cell proliferation rates, in cases of vaginal atrophy expressed and increased after estrogen therapy.

Anthocyanin is a class of flavonoids that have been shown to have phytoestrogen activity both in vivo and invitro. The results of the study found that anthocyanin-rich purple sweet potato ethanol extract was able to increase vaginal epithelium in menopausal animal models. The results of this study show the presence of phytoestrogen activity from ethanol extract of purple sweet potato whose effect is not yet known to the marker of Ki67 proliferation.

This study aims to determine the benefits of giving purple sweet potato ethanol extract at the level of expression of Ki67 mRNA vaginal epithelium of female white rats conducted bilaterally. Female white mice undergo bilateral ovariectomy are used as animal models that must be questioned like menopausal women.

This research is purely experimental with a Post Test Only Control Group Design pattern that aims to understand the effect of ethanol extract of purple sweet potato on Ki67 mRNA vaginal tissue of menopausal animal models. A total of 38 16-week-old female Wistar rats with bilateral ovariectomy were divided into 2 groups, namely the treatment group (P) given purple sweet potato ethanol extract at a dose of 4 ml / day and the control group (K) were given normal saline 4 ml / day for 30 days . On the last day of the study, the experimental animals were terminated, then vaginal tissue was taken to make histological preparations and RNA isolation and qRT-PCR examination of Ki67 were carried out. Descriptive analysis will be presented in graph and table form and completed with ANOVA if it meets the normal and homogeneous distribution requirements using SPSS 16.0 software.

The results of this study will be submitted in the international journal Scopus Q4 and will be submitted to obtain IPR. The TKT of this research is TKT 3 because it is a basic research to prove a concept.