Detection of Genotype gtf-B Streptococcus Mutans in plaque with carious teeth On Students at SD N 29 Dangin Puri

Bacteria that play an important role in the formation of dental plaque as a cause of caries is Streptococcus mutans bacteria. Streptococcus mutans bacteria have glycosyl transferase enzymes that can convert saccharose saliva to extracellular polysaccharide (PSE) through glycosylation process. One of the virulence factors of Streptococcus mutans bacteria as the cause of dental caries is the gengtf-B Streptococcus mutans.

This study aims to detect gen gtf-B Streptococcus mutans in plaque with dental caries in students of SD Negeri 29 Dangin Puri. This study uses 51 children as sample in SD Negeri 29 Dangin Puri who experience caries. Bacterial culture was used to detect colonies of Streptococcus SP. Then, there were performed gram staining, catalase test was also done to distinguish Streptococcus with Staphylococcus. The result of the negative catalase test, PCR Streptococcus Mutans process with size 517 bp and gen gtf B Streptococcus mutans with size 585 bp.

The results of this research found that streptococcus mutans bacteria are as many as 19 samples from 51 samples (37.25%). Three samples from 19 isolates of streptococcus mutans bacteria were detected with gen gtf B streptococcus mutans (16%).