EN 
IN
Journal article
Studying Systematic Errors on Estimation Decision, Detection, and Quantification Limit on Micro-TLC
Gelgel Wirasuta Ni Made Amelia Ratnata Dewi Kadek Duwi Cahyadi Luh Putu Mirah Kusuma Dewi Ni Made Widi Astuti I NYOMAN KADJENG WIDJAJA
Volume : 76 Nomor : 19 Published : 2013, October
Chromatographia
Abstrak
The decision limit (CC?), capability of detection (CC?) and quantification limit (QL) are importance performance characteristics in method validation. The TLC-Scanner 3 from Camag provides the possibility to choose the slit dimension of light to determine the peak chromatogram of a substance. The influence of the slit dimension for determination of CC?, CC? and QL of paracetamol has been carried out. Paracetamol was spotted onto plates of AL-TLC Si G 60 F254 by linomat 4 in the range of 50–400 ng/spot and 10–400 ng/band, then on twin chambers eluted with TAEA (toluene:acetone-ethanol:conc.ammonia, 45 + 45 + 7 + 3 v/v) for 45 mm. Eluted spots were scanned in different slit dimensions at 248 nm. The CC?, CC? and QL of paracetamol were estimated through the linear regression (LRM) and signal-to-noise (S/N) methods. Slit lengths between 50 and 133 % of the band width of the spots, and with the noise factor of the slit under 2.6, produced good precision measurements of TLC-densitometry between plates, while slit lengths between 50 and 83 % of the band width of the spots introduced a higher sensitivity response of the detector. The estimated CC?, CC? and QL were determined by how the data were collected, the analytical optical setting, and the usage method for the estimation of both validation parameters.