Bakteri Escherichia coli Teridentifikasi pada Rektum Lumba-Lumba Hidung Botol Indo-Pasifik di Umah Lumba Rehabilitation Center, Taman Nasional Bali Barat

ABSTRAK Saluran cerna merupakan salah satu tempat pertumbuhan berbagai genus serta spesies bakteri termasuk bakteri family Enterobacteriaceae. Salah satu dari anggotanya adalah Eschericia coli yang merupakan flora normal yang sering ditemukan pada saluran cerna. Saat ini masih sangat langka penelitian tentang keberadaan E. coli pada saluran cerna lumba-lumba hidung botol Indo-Pasifik (Tursiops aduncus). Tujuan penelitian ini adalah untuk mendapatkan data awal mengenai bakteri Enterobateriaceae pada lumba-lumba tersebut yang berada di lingkungan rehabilitasi. Sampel lumba?lumba yang digunakann merupakan lumba-lumba hidung botol Indo-Pasifik (T. aduncus) yang berada di jaring apung laut atau seapen Umah Lumba Rehabititation Center, sebanyak tiga ekor. Pengambilan sampel dilakukan dengan menggunakan rubber catheter sterile berukuran 2,7 mm yang dimasukkan ke dalam rektum lumba-lumba. Jumlah total sampel yang di isolasi sebanyak 12 sampel dari tiga ekor lumba-lumba. Isolasi sampel dilakukan dengan menggunakan media Sheep Blood Agar, media Mac Conkey Agar dan Eosine Methylen Blue Agar. Selanjutnya di uji dengan pewarnaan Gram, uji katalase, uji oksidase, Triple Sugar Iron Agar, Sulfide Indole Motility, Methyl Red Voges Proskauer, Simmon Citrate Agar, dan uji urease. Hasil dari penelitian ini dapat disimpulkan pada tiga ekor lumba-lumba hidung botol Indo-Pasifik (T. aduncus) yang berada di seapen Umah Lumba Rehabilitation Center, ditemukan jenis bakteri famili Enterobacteriaceae spesies E. Coli. Kata-kata kunci: lumba-lumba hidung botol Indo-Pasifik; Tursiops aduncus; Enterobacteriaceae; Escherichia coli ABSTRACT The gastrointestinal tract is one of the growth sites for various genera and species of bacteria, including the Enterobacteriaceae family. One of its members is Eschericia coli which is a normal flora that is often found in the good gastrointestinal tract. Until now, research on the presence of E. coli in the digestive tract of the Indo-Pacific bottlenose dolphin (Tursiops aduncus) is still very rare. The Indonesia Medicus Veterinus Maret 2022 11(2): 234-245 pISSN: 2301-7848; eISSN: 2477-6637 DOI: 10.19087/imv.2022.11.2.234 online pada http://ojs.unud.ac.id/php.index/imv 235 purpose of this study was to obtain preliminary data on Enterobateriaceae bacteria in Indo-Pacific bottlenose dolphins in the rehabilitation environment. The dolphin samples used were the Indo-Pacific bottlenose dolphin (T. aduncus) in the marine floating net or seapen at Umah Lumba Rehabititation Center, as many as three individuals. Sampling was carried out using a sterile 2.7 mm rubber catheter which was inserted into the dolphin's rectum. Rectal samples were taken from three Indo-Pacific bottlenose dolphins with a total of 12 samples isolated. Samples were isolated using Sheep Blood Agar media, Mac Conkey Agar media and Eosine Methylene Blue Agar media. Bacterial identification was then carried out using the Gram stain test, catalase test, oxidase test, Triple Sugar Iron Agar, Sulfide Indole Motility, Methyl Red Voges Proskauer, Simmon Citrate Agar, and Urease test. The results of this study can be concluded in three Indo-Pacific bottlenose dolphins (T. aduncus) residing in the seapen Umah Lumba Rehabilitation Center, positive species of the family Enterobacteriaceae species E. coli. Keywords: Indo-Pasific bottlenose dolphin; Tursiops aduncus; Enterobacteriaceae; Escherichia coli