Inoculation of Helicobacter Pylori Bali03isolate as a Risk Factor in Increasingthe Severity of Gastritis Compared to ATCC 43504 Isolatein Balb/C Mice

Abstract: Gastritis is a common gastrointestinal health problem, where H. pylori infects nearly 50% of the world's population. The prevalence of H. pylori infection in developing countries is higher compared to developed countries. H. pylori causes gastric inflammation in all infected patients. The aim of this study was to find out the mean difference of gastritis degree and prove the degree of gastritis is more severe in BALB/c mice inoculated with H. pylori Bali 03 isolate compared to ATCC 43504 isolate.This study was an experimental study with a randomized posttest only control group design, in 36 male BALB / c mice which were divided into 2 groups : group I was inoculated with H. pylori ATCC 43504isolate, while group II was inoculated with Bali 03 isolates. The study was conducted at the Department of Anatomical Pathology, Sanglah Hospital, Denpasar, and the Biomedical Laboratory of NTB Province Hospital, Mataram, from February to April 2019.In the eighth week, all samples were euthanized, then a gastric resection is performed to assess the gastritis degrees according to revised Sydney system by conventional histopathology and immunohistochemistry.Mice inoculated by H. pylori ATCC 43504 isolate had the incidence of mild and moderate gastritis, 16 (88.8%)and 2 (11.1%) respectively, while mice inoculated by H. pylori Bali 03 isolate had the incidence of mild and moderate gastritis 7 (38.8%)and 11 (61.1%) respectively. The mean difference of gastritis degree showed that the average gastritis score in mice inoculated by H. pylori ATCC 43504 isolate was 3.88; while mice inoculated by H. pylori Bali 03 isolate was 6.22, with p values: 0,000. The difference in the proportion of gastritis degrees withx2 test showed that H. pylori Bali 03 isolate caused severergastritisdegree by 5.5 times compared to H. pylori ATCC 43504 isolate (RR 5.5; 95% IK 1.41-21.37).