First Successful Pregnancy Following Intravagina Insemination Using Frozen Semen in Kintamani Dog

The Kintamani dog is a native Balinese dog that has been designated as a temporary category breed (breeds recognized on a provisional basis) by the Federation Cynologique Internationale since 2019. To become a definitive breed, it takes ten years to improve its quality and population size. If this was not achieved, the Kintamani dog was removed from the breed category. Therefore, efforts must be made in the future to increase the population and genetic quality of Kintamani dogs. This study aimed to provide frozen semen of Kintamani dogs for rapid improvement of the quality and population of Kintamani dogs with the specific target of evaluating the fertilization capacity of frozen semen of Kintamani dogs using the artificial insemination method. Eight bitches were used in this experiment. The dog was in good health. The time of artificial insemination was performed 11 days after the onset of proestrus. Bitches are inseminated intravaginally. Pregnancy was confirmed by ultrasound 25 days post-AI. To our knowledge, this is the first successful case of AI in a Kintamani dog. From these results, it can be concluded that freezing the semen of Kintamani dogs could fertilize female dog eggs.