Case of Entamoebiasis in Pigs Raised with a Free Range Systems in Bali, Indonesia

This research aims were to measure the prevalence of Entamoeba in pigs in Bali and to identify the zoonotic potential species of Entamoeba. A total of 183 pig stool samples from Bali have been examined. The method being used in this study were combination between coproscopy and molecular techniques. Concentration sedimentation with Sodium Acetic Formaldehide (SAF) as a solution was used in the coproscopy method, while the Polimerase Chain Reaction method was used to amplify DNA of Entamoeba. Extracted sample’s DNA examined by using primers that specifically for Entamoeba: Entam 1 (F) (5’-GTT GAT CCT GCC AGT ATT ATA TG-3’) and Entam 2 (R) (5’-CAC TAT TGG AGC TGG AAT TAC-3), and to identify the zoonotic potential species of Entamoeba, samples that produce 550 bp in first amplification continued by primers Epolecki1 (F) (5’-TCG ATA TTT ATA TTG ATT CAA ATG-3’) and Epolecki2 (R) (5’- CCT TTC TCC TTT TTT TAT ATT AG-3’). The results showed that 76.6% of samples were positive in coproscopical examination, but 84.7 % produced 550 bp bands on PCR amplification by using general primers. All positive samples on the first PCR continued to second PCR used specific primers for E. poleckii as a potential zoonotic disease and all of the samples showed negative results. This data demonstrated that the prevalence of Entamoeba in a traditional pig scavenging systems in Bali was 84.7% but no specific infection infection caused by E. polecki was found.