Deteksi Virus Classical Swine Fever di Bali dengan RT-PCR

Classical Swine Fever (CSF) virus has been confirmed for the first time in pig in Bali. The object of this study was suspected CSF cases diagnosed at the diagnostic laboratory assistantship of the Faculty of Veterinary Medicine, Udayana University, in 2007-2008. Total number of cases was 12. Case records included the signalment of case (breed, age, body weight, and the origin of respective case), clinical signs, post-mortem lesions, and histological pictures. CSF virus was confirmed using the standardized reverse transcriptase-polymerase chain reaction (RT-PCR) for CSF from European Union. One RT-PCR product was sequenced. CSF virus was confirmed in seven out of 12 cases (58%). The cDNA sequence was confirmed to be specific of CSF E2 protein coding region with 98% homology to one isolate from China that was available in GeneBank. Further works are recommended to elucidate the sensitivity of RT-PCR, to clarify some differential diagnose, and to find out the genetic variation of CSF virus in Bali.