Cemaran Timah Hitam dalam Darah Sapi Bali yang Dipelihara Di Tempat Pembuangan Akhir Kota Denpasar

bstrak Sapi bali terkenal memiliki daya adaptasi tinggi terhadap lingkungan, terbukti dapat hidup di lingkungan kritis termasuk di tempat pembuangan akhir (TPA). Penelitian ini bertujuan untuk mengetahui hubungan aktivitas serum glutamate pyruvate transaminase (SGPT) dan serum glutamate oxaloacetat transminase (SGOT) dengan kadar timah hitam/plumbum (Pb) dalam darah sapi bali yang dipelihara di TPA Kota Denpasar. Sampel sapi yang digunakan dalam penelitian ini berjumlah 22 ekor. Darah diambil dari vena jugularis masing-masing sebanyak 10 mL yang ditampung ke dalam tabung berisi EDTA 0,5% dan 10 mL pada tabung non EDTA. Pengukuran kadar SGPT dan SGOT dilakukan dengan menggunakan metode Auto analizer (Refloton(R) plus), sedangkan pengukuran logam berat Pb dalam plasma darah, dilakukan dengan metode atomic absorption spektrofotometry (AAS). Hasil pengukuran SGPT dan SGOT diperoleh hasil yang sangat bervariasi dengan rataan SGPT= 29,96±5,67 U/L dan SGOT= 78,82±17,62 U/ L Demikian pula hasil pengukuran kadar Pb sangat bervariasi antara 4,004 sampai 10,216 ppm dengan rataana 6,59±1,85 ppm. Analisis korelasi menunjukkan adanya hubungan antara aktivitas SGOT dengan kadar Pb, tetapi tidak ada korelasi antara kada SGPT dengan kadar Pb. Hasil ini menunjukkan bahwa cemaran Pb dapat menyebabkan gangguan patologi sel-sel jaringan termasuk hati. Disimpulkan terdapat cemaran logam berat Pb dalam darah sapi bali yang dipelihara di TPA Kota Denpasar, yang terdistribusi pada berbagai jaringan. Kata-kata kunci : daya adaptasi; SGPT/SGOT; logam berat Pb. Abstract Bali cattle are known for having high adaptability to any condition at environment, proved to be living in critical environments including landfills (TPA). This study aim was to determine the relationship between SGPT/SGOT levels and lead (Pb) level in blood of bali cattle reared in the landfill Denpasar. A total of 22 bali cattles were used in this study was. The blood was drawn from the jugular vein and 10 ml blood were collected using tubes containing EDTA 0.5% and 10 mL without EDTA tubes. Levels of SGPT and SGOT were measured by using Auto analyzer (Refloton (R) plus) method, while the measurement of Pb in blood plasma was conducted by using atomic absorption spectrophotometry (AAS) method. Result of the SGPT and SGOT measurement obtained were relatively varied with an average of SGPT = 29.96±5.67 U/L and SGOT = 78.82±17.62.U/L. Similarly, Pb measurement results vary widely between 4.004 to 10.216 with an average of 6.595±1.85 ppm. Correlation analysis showed that there was an association between SGOT with Pb levels. However, no correlation was observed between the SGPT with Pb levels. These results indicate that the levels of lead may cause pathological tissue cells in organs other than liver. Its concluded that heavy metal Pb is found in the blood of bali cattle reared in Denpasar city landfill, and it may be correlated with the level of the SGOT and may cause pathological tissue cells organs. Key words: adaptability, SGPT / SGOT, heavy metals Pb,