Correlation between leptin and LH concentration during peri and postpartum to the onset of estrus postpartum in Bali cattle

Mekanisme poros hipotalamus, hipofisis dan ovarium berperan penting pada awal aktivitas luteal postpartum. Penelitian ini bertujuan untuk mengukur kadar hormon leptin, Luteinizing Hormon (LH) saat partum dan saat munculnya estrus pertama postpartum serta melihat munculnya tanda-tanda estrus. Rancangan penelitian yang digunakan adalah observasional prospektif. Sampel yang digunakan adalah sapi Bali pada saat partus dan saat munculnya estrus pertama postpartum yang ada pada beberapa system pertanian terintegrasi (simantri) di Kecamatan Mengwi, Kabupaten Badung, Provinsi Bali. Parameter yang diukur adalah kadar leptin, LH dan waktu munculnya estrus postpartum. Teknik pengukuran kadar hormon dilakukan dengan metode Direct Enzyme-linked immunosorbent assay (ELISA), Double Antibody Sandwich. Hasil penelitian menunjukkan terdapat korelasi antara kadar leptin saat partus dengan waktu munculnya estrus postpartum dengan nilai korelasi (r) = 0,773. Hasil penelitian menunjukkan bahwa tingginya kadar leptin saat partus waktu munculnya estrus postpartum yang lebih cepat. Namun diperlukan tingkat minimum dari kadar leptin untuk meningkatkan kadar LH sehingga meningkatkan ekspresi munculnya estrus. Kata kunci : Leptin peripartum, LH, estrus postpartum ABSTRACT The mechanism of the hypothalamus, pituitary, and ovaria is important in the early postpartum luteal activity. This study aims to measure levels of the leptin hormone, Luteinizing Hormone (LH) during the first-time postpartum estrus appears and to observe the signs of estrus. The study uses a prospective observational method. The samples were Bali cattle on peripartum condition while postpartum estrus appeared from several sistem pertanian terintegrasi (Simantri) in Mengwi District, Badung Regency, Bali, Indonesia. The measured parameters were leptin levels, LH, and the onset of postpartum estrus. The technique of measuring hormone levels with the Direct Elisa method and Double Antibody Sandwich. The results showed there were a positive correlation (r=0.773) between leptin levels at peripartum and the onset of postpartum estrus.The higher level of leptin, the faster of postpartum estrus appears. However, a minimum leptin value is needed to increase the LH level so thatexpression of estrus appearance is clearly. Keywords: peripartum Leptin, LH, postpartum estrus