Genetic Diversity of Gembrong Goat Based on DNA Microsatellite Markers

The present study was undertaken with primary objective to characterize Gembrong goat breeds. It is essential to characterize the germplasm for intragenetic variability, which will help in planning for conservation strategy as well as genetic improvement. DNA genome was isolated from hairs. Fourteen microsatellite were amplified by PCR. PCR product were run on 6% bis-Acrylamide gel in automated DNA sequencer. The result showed that all markers successfully amplified in Gembrong goat microsatellite loci. All the investigated microsatellites were found to be highly polymorphic, except SCRSP23, INRA063 and CSRD247. A total of 32 alleles were observed in the whole samples and the number of alleles per locus varied from one 1 (SCRSP23, INRA063 and CSRD247) to 5 (HSC) alleles. The allele sizes ranged from 85 bp (SCRSP23) to 302 bp (HSC). The PIC values ranged from 0,00 (locus SCRSP23, INRA063 and CSRD247) to 6162 (locus HSC). The average observed and expected heterozygosities ranged from 0.00 (locus SCRSP23, INRA063 and CSRD247) to 0,7308 (locus HSC) and 0.00 to 0,6765 (locus HSC), respectively