Detection of Mycobacterium bovis and Klebsiella pneumoniae at Bali Cattle Slaughterhouse by culture analysis and PCR

ABSTRAK Penelitian mengenai prevalensi bovine tuberculosis dan Klebsiella pneumoniae pada rumah potong sapi di Bali dilakukan di Rumah Poyong Pesanggrahan dan Mambal dari Bulan Januari sampai dengan Maret 2015. Pengamatan terhadap lesi dilakukan pada organ paruparu dan limfenodus yang selanjutnya diperiksa melalui analisis DNA untuk Mycobacterium bovis dan kultur untuk isolasi Klebsiella pneumoniae. Dari seluruh sampel, tidak ada sampel yang ditemukan lesi yang mengidikasikan menderita tuberculosis di kedua rumah potong hewan. Namun, Klebsiella pneumoniae teramati pada 1/4513 sampel (0,02%) hanya di Rumah Potong Sap Mambal. Penelitian ini merupakan laporan pertama adanya Klebsiella pneumoniae di Bali. Karenanya, dibutuhkan kehati-hatian terhadap Klebsiellosis yang besifat zoonotik ini dan formulasi management kontrolnya. Kata kunci: sapi Bali, Mycobacterium bovis, Klebsiella pneumoniae, prevalensi ABSTRACT A study to determine the presence and prevalence of bovine tuberculosis and Klebsiella pneumoniae at cattle slaughterhouse in Bali was carried out in Pesanggaran and Mambal abattoirs from January to March 2015.The Lungs and lymph nodes were inspected for lesions and then examined through DNA analysis for Mycobacterium bovis and culture for isolation of Klebsiella pneumoniae. Of the samples examined, no one had lesions suggestive of tuberculosis in two abattoirs.However, Klebsiella pneumoniae was observed 1/4513 sample (0.02%) only in Mambal abattoir. This study is the first report of the presence of Klebsiella pneumoniae in Bali. Therefore, a aware of this zoonotic Klebsiellosis and its control management formulation are need. Keywords : Bali Cattle, Mycobacterium bovis, Klebsiella pneumoniae, prevalence