Suplementasi Mineral Pada Pakan Sapi Bali Terhadap Diferensial Leukosit Di Empat Tipe Lahan

The aims of this research were to know the effect of mineral intake on Balinese cattle that has been fed on four different types of land (rice field, grassland, garden, and forest) towards the leukocytes differential. The research design follows a completely randomized design (CRD) using a nested pattern on 48 males from Balinese cattle. The cattle were grouped according to four treatments of mineral intake, namely: controls, 2.5 grams, five grams, and 7.5 grams in each type of land. Furthermore, the blood was taken from the auricularis superficialis vein in the third month after treatment. A blood smear was prepared using the slide method and a sample was stained with Giemsa. The blood smear was examined by leukocytes differential using battlement method with 1000x magnification. Then, the collected data were analyzed using analysis of variance and Cochran test. The research results show that the given treatment of minerals has no change the leukocytes differential. In contrast, the percentage of lymphocytes in the type of garden land has lower than on the type of forest, rice land and grassland, but has no differences the percentage of neutrophils, eosinophils, basophils, and monocytes in the four types of land. The conclusion is giving to a concentration of 7.5 gram does not change the differential leukocyte Bali cattle.