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IN
Journal article
Mikropropagasi Anggrek Phalaenopsis dengan Menggunakan Eksplan Tangkai Bunga
Hestin Yuswanti I PUTU DHARMA Utami I WAYAN WIRAATMAJA
Volume : 5 Nomor : 2 Published : 2015, November
Agrotrop Journal on Agriculture Science
Abstrak
Micropropagation of Phalaenopsis Orchid Using Explants of Flower Stalk. This study aimed to obtain optimum media formulations for bud multiplication of micropropagation of Phalaenopsis orchid using flower stalk. This study employed Completely Randomized Design with 5 different treatments, i.e. M0 = MS + 150 ml/l coconut water + 2 g activated charcoal, M1 = MS + 1 ppm IBA+ 1 ppm BAP + 150 ml/l coconut water + 2 g/l activated charcoal M2 = MS + 1ppm IBA+ 2 ppm BAP+ 150 ml/l coconut water + 2 g/l activated charcoal, M3 = MS + 1 ppm IBA+ 3 ppm BAP + 150 ml/l coconut water + 2 g/l activated charcoal, M4 = MS + 1 ppm IBA+ 4 ppm BAP + 15 0ml/l coconut water + 2 g/l activated charcoal. Each treatment consists of 5 replicates, so there were 25 units experiment, with 3 explants each. Results of this study revealed that M2 media exhibited the best growth, as shown by swelling buds (23.77 Days After Planting/DAP), earliest buds formation (49.33 DAP) and height of buds of 2,12cm.