Utilization of Phosphate Solubilizing Rhizobacterium Derived from Leguminosae Plants to Stimulating Plant Growth and Induce Systemic Resistance of Soybean (Glycine max l. Merrill) to Mosaic Diseases

In Indonesia, soybeans are grown in paddy fields after rice harvesting, the production is much lower compared to soybean planted on dry land it is caused by the growth of roots and plants, is not good caused by former land of rice plants is often flooded, a result the soil structure becomes solid, less oxygen, microorganism population is low, and most of the nutrients are still bound in soil granular and poorly absorbed by plant roots. Additionally soybean planting in paddy fields, susceptible to pests and diseases, because of lack of nutrients for plants produce secondary metabolites that can protect plants from pathogens. Therefore conducted research in order to obtain phosphate solubilizing rhizobacterium (PSR) from Rhizosfera of the Leguminosae plants capable of inducing growth of Rhizobium on soybean root. So expect the soybean plants will get enough Nitrogen from the Rhizobium and Phosphate from PSR activity. Isolation of PSR will be isolated from the roots of Leguminosae plants besides soybean in the all districts of Bali. From the results of isolation was obtained 147 isolates Rhizobakteria, after study its ability to stimulate the growth of soybean roots, obtained 58 isolates were able to stimulate root growth. Of the 58 rhizobacteria isolates was tested his ability as a Phosphate solubilizing, was only obtained 14 PSR. Isolates as phosphate solubilizing highest were produced by Rhi 36 and Rhi 35 isolates, both PSR was isolated from roots undis (Cajanus cajan) with dilutions index respectively 8.80 and 7.75, while the lows phosphate dilution index was produced by isolates Rhi 52, from Solanum nigrum, with 0.06 dissolution Index. Rhi 36 isolates, is the best isolates to increase vegetative soybean plant growth. To improve the generative growth is best to Rhizobacteria isolate of Rhi 9 from Leucaena glauca roots. Phosphate solubilizing rhizobacterium as a potential inducer of systemic resistance against virus pathogen of soybean such as Potyvirus and Cucumber Mosaic Virus (CMV) is Rhi 6 isolate from agathi root plants (Sesbania grandiflora), Rhi 53 from Pigeon pea plant (Cajanus cajan) and Rhi 3 from Sword Jack Bean plants (Stylosanthes guianensis). The all three isolates induces systemic plant resistance against the virus by stimulating the plant to produced chemical compounds such as phenol, salicylic acid and peroxidase.