Pengaruh NAA-BAP dan Jenis Eksplan pada Mikropropagasi Anggrek Vanda tricolor Lindl.

ABSTRACTS The Effect of Combination of BAP-NAA and Explant Types on Micropropagation of Orchid of Vanda tricolor Lindl. var. suavis The objectives of the research was to investigate the effect of combination of NAA–BAP, type of explant and their interactions on shoot initiation in micropropagation of Vanda tricolor L. var. suavis. The factorial experiment consisted of two factors that was arranged by completely randomized design (CRD) with three replications. The first factor was combination of NAA-BAP i.e. M0= MS medium without any plant growth regulator; M1= MS + 0,1 mgL-1 NAA + 0 mgL-1 BAP; M2= MS + 0,1 mgL-1 NAA + 0,1 mgL-1 BAP; M3= MS + 0,1 mgL-1 NAA + 1 mgL- 1 BAP ; and M4= MS + 0,1 mgL-1 NAA + 10 mgL-1 BAP. The second factor was explant type i.e. shoot tip (B1) and shoot base (B2). The results showed that there was no interaction between factors for all variable observed. Medium M0 (MS medium without any plat growth regulator) and explant of B1 (shoot tip) gave the highest number of the percentage of explants producing shoots and the highest number of shoots per explant. Keywords: micropropagation, NAA -BAP, V. tricolor Lindl. var. suavis, shoot initiation, explant types