ISOLASI DAN IDENTIFIKASI PATOGEN GETAH KUNING MANGGIS MELALUI PENDEKATAN POSTULAT KOCH DAN ANALISIS SECARA MOLEKULER

Isolation and identification of mangosteen yellow latex through Koch’s postulate application and molecular analysis. The yellow latex disease decreases fruit quality of mangosteen because of exogenic latex causes dirty fruit and the endogenic latex makes the bitter fruit taste and does not deserve to be consumed. The causal agent of the disease was fisiologically disorder that was the broken cell due to unstability of cell turgor. However, the drift irrigation treatment was just able to decreases the desease of 35.22%, therefore the causal agent could be microbe that promote the yellow latex. This research was aimed to find the yellow latex pathogen/s with its characteristics. The Koch Postulate was apllied to isolate and proved the microbes associated to the yellow latex and molecular analysis was constructed subsequently to find the microbe species. The result of the research proved that there were three fungus as the biotic agent responsible to the disease. The fungus were Verticillium albo-atrum with the highest pathogenecity of 74.87%, followed by Fusarium oxysporum of 70.15%, and Pestalotia macrotricha with pathogenecity of 20.32%. Key words: yellow latex, Postulate Koch, biotic factor.