Combined Effects of Noni Fruit Extract (Morinda Citrifolia L.) and Warfarin on Bleeding and Coagulation Time of Mice

Background and Objectives : Noni (Morinda Citrifolia L.) fruit has been studied for its platelet antiaggregation effect. Warfarin is one of the synthetic derivative of coumarin compounds, which is regulary use as an oral anticoagulant. There is a possibility of potentiation platelet antiaggregation activity which is characterized by bleeding and coagulation time. The objectives of this research is to study combined effect of warfarin and noni fruit extract to mice’s bleeding and coagulation time. Methods : Bleeding time was determined through tail bleeding method. Coagulation time was determined through cappillary pipe method. Mice were randomly divided into 3 groups. Group 1 received noni fruit extract 100 mg / kg bw once daily, group 2 received warfarin 1 mg / kg bw once daily, group 3 received warfarin 1 mg / kg bw + noni fruit extract 100 mg / kg bw once daily, each given for 7 days. Bleeding time and coagulation time were tested twice, once before being treated and then on day-7 treatment. Result : Result of this study showed that bleeding and coagulation time was significantly increased after treatment. Group 1 which received noni fruit extract 100 mg/kg bw, bleeding time increased from 52.75 + 1.60 seconds to 111.00 + 4.45 seconds and coagulation time from 60.00 + 4.01 seconds to 101.25 + 3.75 seconds. Group 2 which received warfarin 1 mg/ kg bw, bleeding time increased from 56.62 + 1.71 seconds to 152.88 + 5.86 second and coagulation time from 60.00 + 4.01 seconds to 136.88 + 4.43 seconds. Group 3 which received combination of noni fruit extract 100 mg/kg bw and warfarin 1 mg/kg bw, bleeding time increased from 56.00 + 1.43 seconds to 236.38 + 4.24 seconds and coagulation time from 63.75 + 3.75 seconds to 213.75 + 8.85 seconds. Conclusion : Combination of noni fruit extract and warfarin can prolonged bleeding and coagulation time of mice’s.